The story picks up a few months after the end of the first season. Youkai Gakuen has been rebuilt, and the new semester is underway. Tsukune and his group return, now more powerful than ever. The true identity of Anti-Thesis is revealed. New enemies appear on the horizon, along with something far more sinister than anything the gang has faced before.
Tsukune struggles with the monster growing inside him. With help from Moka, Mizore, Kurumu, Ruby, and Yukari, he trains to control his vampire blood and gain the strength to protect what he cares about.
This season focuses more on the girls, their pasts, and their families. New confrontations lie ahead. New friends and allies will join them. Together, they will uncover the secrets of Moka's rosary and her lineage.